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Anti-beta Tubulin

Boster Bio Anti-beta Tubulin (Catalog # M32657) . Tested in WB, Flow Cytometry, IF application (s) . This antibody reacts with Mouse, Rat.

Product Specifications

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Synonyms

Tubulin beta-5 chain, Tubb5

Gene Name

Tubb5

UniProt

P99024

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Expressed in embryonic brain, including throughout the developing cortex and in the subventricular zone. Also found in radial glial cells, intermediate progenitors, migrating neurons and postmitotic neurons (PubMed:23246003) . Expressed in skin and developing hair follicle (PubMed:26637975)

Applications

WB, Flow Cytometry, IF

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

49671 Da

Subcellular Location

Cytoplasm, cytoskeleton

Prediction Reactivity

C. elegans, Chicken, Drosophila, Human, Monkey, Bovine, Xenopus, Hamster, Pig

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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