Anti-TFIIB monoclonal GTF2B Antibody

Expressed in the inner cell mass forming the embryoblast (PubMed:24441171) . Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171) .

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II) . Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682) . Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312) . Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352) . Associates with chromatin to core promoter- specific regions (PubMed:12931194, PubMed:24441171) . Binds to two distinct DNA core promoter consensus sequence elements in a TBP- independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841) . Modulates transcription start site selection (PubMed:10318856) . Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194) .