Anti-H3f3b Antibody (C-Term)
Boster Bio Anti-H3f3b Antibody (C-Term) (Catalog # M06819-30) . Tested in WB application (s) . This antibody reacts with Mouse.
Product Specifications
Background
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms
Histone H33, H3f3a, H33a
Gene Name
H3-3a
UniProt
P84244
Host
Rabbit
Reactivity
Mouse
Immunogen
This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-136 amino acids from mouse H3f3b.
Clonality
Polyclonal
Applications
WB
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
15328 Da
Applications Notes
WB: 1:2000
Subcellular Location
Nucleus. Chromosome.
Isotype
Rabbit IgG
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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