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Anti-ATG4A Antibody

Boster Bio Anti-ATG4A Antibody (Catalog # M06539-1) . Tested in WB, Flow Cytometry, IHC-P, IF application (s) . This antibody reacts with Human.

Product Specifications

Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Synonyms

Cysteine protease ATG4A, 3422-, AUT-like 2 cysteine endopeptidase, Autophagin-2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, hAPG4A, ATG4A, APG4A, AUTL2

Gene Name

ATG4A

UniProt

Q8WYN0

Host

Mouse

Reactivity

Human

Immunogen

This ATG4A antibody is generated from a mouse immunized with a recombinant protein.

Clonality

Monoclonal

Clone

Clone: 1458CT808.66.25.69

Applications

WB, Flow Cytometry, IHC-P, IF

Field of Research

Cancer, Cardiovascular, Cell Biology, Metabolism, Autophagy

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

45378 Da

Subcellular Location

Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}.

Isotype

IgG2b, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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