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Anti-USP25 Antibody

Boster Bio Anti-USP25 Antibody (Catalog # M06182) . Tested in WB application (s) . This antibody reacts with Human.

Product Specifications

Background

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.

Synonyms

Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21

Gene Name

USP25

UniProt

Q9UHP3

Host

Mouse

Reactivity

Human

Immunogen

This USP25 antibody is generated from a mouse immunized with a recombinant protein from human USP25.

Clonality

Monoclonal

Clone

Clone: 1277CT376.106.171

Tissue Specificity

Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific

Applications

WB

Field of Research

Cell Biology

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

122218 Da

Applications Notes

WB: 1:1000

Subcellular Location

Cytoplasm

Isotype

IgG1, κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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