Anti-GAPDHS Purified Monoclonal Antibody
Boster Bio Anti-GAPDHS Purified Monoclonal Antibody (Catalog# M06121) . Tested in WB, ICC, Flow Cytometry application (s) . This antibody reacts with Pig, Human.
Product Specifications
Background
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Synonyms
SGAPDH, HSD-35
Gene Name
GAPDHS
UniProt
O14556
Host
Mouse
Reactivity
Pig, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
Clonality
Monoclonal
Clone
Clone: Hs-8
Tissue Specificity
Testis specific.
Applications
WB, ICC, Flow Cytometry
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .
Concentration
1 mg/mL
Form
Liquid
Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity) . .
Storage Conditions
Store at 2-8°C. Do not freeze.
Observed Molecular Weight
44501 MW
Gene Name Synonym
Glyceraldehyde-3-phosphate dehydrogenase, spermato
Subcellular Location
Cytoplasm.
Protein Name
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific
Isotype
Mouse IgM
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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