Anti-USP5 Antibody
Boster Bio Anti-USP5 Antibody (Catalog # M04550) . Tested in WB application (s) . This antibody reacts with Human, Mouse, Rat.
Product Specifications
Background
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Synonyms
Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5, USP5, ISOT
Gene Name
USP5
UniProt
P45974
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.
Clonality
Monoclonal
Clone
Clone: 1340CT704.170.140
Applications
WB
Field of Research
Cell Biology
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Function
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys- 48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
95786 Da
Applications Notes
WB: 1:2000
Isotype
IgG1, κ
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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