Recombinant Human Cardiac muscle troponin T
Product Specifications
Background
Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.
Gene ID
7139
UniProt
P45379
Target
SDIEEVVEEY EEEEQEEAAV EEEEDWREDE DEQEEAAEED AEAEAETEET RAEEDEEEEE AKEAEDGPME ESKPKPRSFM PNLVPPKIPD GERVDFDDIH RKRMEKDLNE LQALIEAHFE NRKKEEEELV SLKDRIERRR AERAEQQRIR NEREKERQNR LAEERARREE EENRRKAEDE ARKKKALSNM MHFGGYIQKQ AQTERKSGKR QTEREKKKKI LAERRKVLAI DHLNEDQLRE KAKELWQSIY NLEAEKFDLQ EKFKQQKYEI NVLRNRINDN QKVSKTRGKA KVTGRWK
Purification
> 98 % by SDS-PAGE and HPLC analyses.
Endotoxin
Less than 1 EU/ug of rHucTnT as determined by LAL method.
Bioactivity
Data not available.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
Calculated Molecular Weight
Approximately 35.8 kDa, a single non-glycosylated polypeptide chain containing 297 amino acids.
Formulation
Supplied as a 0.2 μm filtered solution in 10 mM Tris-HCl, pH8.0, 250 mM NaCl, with 50% glycerol.
Host or Source
Escherichia coli
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