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Recombinant Human Cardiac muscle troponin T

Product Specifications

Background

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Gene ID

7139

UniProt

P45379

Target

SDIEEVVEEY EEEEQEEAAV EEEEDWREDE DEQEEAAEED AEAEAETEET RAEEDEEEEE AKEAEDGPME ESKPKPRSFM PNLVPPKIPD GERVDFDDIH RKRMEKDLNE LQALIEAHFE NRKKEEEELV SLKDRIERRR AERAEQQRIR NEREKERQNR LAEERARREE EENRRKAEDE ARKKKALSNM MHFGGYIQKQ AQTERKSGKR QTEREKKKKI LAERRKVLAI DHLNEDQLRE KAKELWQSIY NLEAEKFDLQ EKFKQQKYEI NVLRNRINDN QKVSKTRGKA KVTGRWK

Purification

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin

Less than 1 EU/ug of rHucTnT as determined by LAL method.

Bioactivity

Data not available.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

Calculated Molecular Weight

Approximately 35.8 kDa, a single non-glycosylated polypeptide chain containing 297 amino acids.

Formulation

Supplied as a 0.2 μm filtered solution in 10 mM Tris-HCl, pH8.0, 250 mM NaCl, with 50% glycerol.

Host or Source

Escherichia coli

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