Anti-TRIM33 (C-terminus) monoclonal Antibody
Boster Bio Anti-TRIM33 (C-terminus) monoclonal Antibody catalog # M03133. Tested in WB applications. This antibody reacts with Human.
Product Specifications
Synonyms
E3 ubiquitin-protein ligase TRIM33;2.3.2.27; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; RING-type E3 ubiquitin transferase TRIM33 ; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; TRIM33; KIAA1113, RFG7, TIF1G
Gene Name
TRIM33
UniProt
Q9UPN9
Host
Mouse
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to the C-terminus of Human TRIM33.
Clonality
Monoclonal
Tissue Specificity
Expressed in stem cells at the bottom of the crypts of the colon (at protein level) . Expressed in colon adenomas and adenocarcinomas (at protein level) . Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
Applications
WB
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity) . Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade) .
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
122533 MW
Applications Notes
WB: 1:1000
Gene Name Synonym
E3 ubiquitin-protein ligase TRIM33
Subcellular Location
Nucleus
Protein Name
E3 ubiquitin-protein ligase TRIM33
Contents
1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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