Anti-HIF1AN Antibody (C-term)

Boster Bio Anti-HIF1AN Antibody (C-term) (Catalog # M02116-1) . Tested in WB, IF application (s) . This antibody reacts with Human.

Product Specifications

Background

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD) . Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Synonyms

Hypoxia-inducible factor 1-alpha inhibitor, 1.14.11.30, 1.14.11.n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1

Gene Name

HIF1AN

UniProt

Q9NWT6

Host

Mouse

Reactivity

Human

Immunogen

This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN.

Clonality

Monoclonal

Clone

Clone: 931CT3.1.1

Applications

WB, IF

Field of Research

Cancer, Cardiovascular

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD) . Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

40285 Da

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages