Anti-HIF1AN Antibody (C-term)
Boster Bio Anti-HIF1AN Antibody (C-term) (Catalog # M02116-1) . Tested in WB, IF application (s) . This antibody reacts with Human.
Product Specifications
Background
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD) . Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Synonyms
Hypoxia-inducible factor 1-alpha inhibitor, 1.14.11.30, 1.14.11.n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1
Gene Name
HIF1AN
UniProt
Q9NWT6
Host
Mouse
Reactivity
Human
Immunogen
This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN.
Clonality
Monoclonal
Clone
Clone: 931CT3.1.1
Applications
WB, IF
Field of Research
Cancer, Cardiovascular
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Function
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD) . Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
40285 Da
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages
Isotype
IgG1, k
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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