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Anti-MBD2 Antibody

Boster Bio Anti-MBD2 Antibody (Catalog # M01746-1) . Tested in WB, IHC-P application (s) . This antibody reacts with Human.

Product Specifications

Background

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Synonyms

Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2

Gene Name

MBD2

UniProt

Q9UBB5

Host

Mouse

Reactivity

Human

Immunogen

This MBD2 antibody is generated from a mouse immunized with a recombinant protein between 10-228 amino acids from human MBD2.

Clonality

Monoclonal

Clone

Clone: 1812CT625.9.45

Tissue Specificity

Highly expressed in brain, heart, kidney, stomach, testis and placenta.

Applications

WB, IHC-P

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

97746691094785224307175104714991094785216428440289776661047149910947852164151791641517912665568

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

43255 Da

Subcellular Location

Nucleus. Chromosome Note=Nuclear, in discrete foci (PubMed:12183469) . Detected at replication foci in late S phase. Localizes to methylated chromatin (PubMed:16428440) . Localizes to sites of DNA damage in a manner partially dependent on ZMYND8 (PubMed:27732854)

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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