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Anti-SPI1 Antibody

Boster Bio Anti-SPI1 Antibody (Catalog # M01116-1) . Tested in WB, IHC application (s) . This antibody reacts with Human.

Product Specifications

Background

Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) .

Synonyms

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

Gene Name

SPI1

UniProt

P17947

Host

Mouse

Reactivity

Human

Immunogen

This SPI1 antibody is generated from a mouse immunized with a recombinant protein of human SPI1.

Clonality

Monoclonal

Clone

Clone: 1835CT291.10.52

Tissue Specificity

In the bone marrow, concentrated in hematopoietic stem cell, lymphoid progenitor, myeloid lineage (granulocyte macrophage progenitors, classical dendritic cells, monocytes) and B-cell clusters Among B-cells, predominantly expressed in pre-B1 cells (PubMed:33951726) . Expressed in germinal center B-cells (PubMed:23166356) .

Applications

WB, IHC

Field of Research

Immunology, Interleukins

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

23658224339517263395172633951726

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

31083 Da

Subcellular Location

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00237, ECO:0000269|PubMed:33951726}

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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