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Anti-MGMT Antibody

Boster Bio Anti-MGMT Antibody (Catalog # M01012-2) . Tested in WB, Flow Cytometry application (s) . This antibody reacts with Human.

Product Specifications

Background

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Synonyms

Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT

Gene Name

MGMT

UniProt

P16455

Host

Mouse

Reactivity

Human

Immunogen

This MGMT antibody is generated from a mouse immunized with a recombinant protein.

Clonality

Monoclonal

Clone

Clone: 1527CT158.87.44

Applications

WB, Flow Cytometry

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

21646 Da

Subcellular Location

Nucleus.

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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