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Anti-MAD2L1/Mad2 Monoclonal Antibody

Boster Bio Anti-MAD2L1/Mad2 Monoclonal Antibody (Catalog # M00785) . Tested in IP, WB applications. This antibody reacts with Human.

Product Specifications

Background

MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14.

Synonyms

MAD2L1, Mitotic arrest deficient yeast homolog like 1, Mitotic spindle assembly checkpoint protein

Gene Name

MAD2L1

UniProt

Q13257

Host

Mouse

Reactivity

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.

Clonality

Monoclonal

Clone

Clone: Clone: 17D10 IgG1

Applications

ELISA, IP, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Division, Nucleus, Spindle, Subcellular Markers, Tags & Cell Markers

Purification

This Protein A purified antibody is directed against human MAD2L1 protein. The product was purified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity against homologues from other sources is not known.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

23510 MW

Gene Name Synonym

Mitotic spindle assembly checkpoint protein MAD2A

Subcellular Location

Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable) . Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.

Isotype

IgG1

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

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