Anti-RAD51 Antibody

Boster Bio Anti-RAD51 Antibody (Catalog # M00088-2) . Tested in WB application (s) . This antibody reacts with Human.

Product Specifications

Background

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Synonyms

DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A, RAD51, RAD51A, RECA

Gene Name

RAD51

UniProt

Q06609

Host

Mouse

Reactivity

Human

Immunogen

This RAD51 antibody is generated from a mouse immunized with a recombinant protein between 1-339 amino acids from human RAD51.

Clonality

Monoclonal

Clone

Clone: 1690CT361.33.68

Tissue Specificity

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast

Applications

Field of Research

Mitochondria

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

36966 Da

Applications Notes

WB: 1:4000

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage (PubMed:20154705) . DNA damage induces an increase in nuclear levels (PubMed:20154705) . Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment (PubMed:23754376) . Accumulated at sites of DNA damage in a SPIDR- dependent manner (PubMed:23509288) . Recruited at sites of DNA damage in a MCM9-MCM8-dependent manner (PubMed:23401855) . Colocalizes with ERCC5/XPG to nuclear foci in S phase (PubMed:26833090) . Recruited to stalled replication forks during replication stress by the TONSL-MMS22L complex, as well as ATAD5 and WDR48 in an ATR-dependent manner (PubMed:27797818, PubMed:31844045) .