Anti-GSG2 Antibody (Center)

Boster Bio Anti-GSG2 Antibody (Center) (Catalog # A31862-1) . Tested in IHC-P, WB application (s) . This antibody reacts with Human.

Product Specifications

Background

Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Mitotic phosphorylation of H3 Thr 3 occurs in prophase and dephosphorylation during anaphase. Haspin, a dual serine/threonine kinase, plays an important role in regulation of chromosome and spindle function during mitosis and meiosis via its function in phosphorylation of the threonine residue in the third position of histone 3 (Thr3) .

Synonyms

Serine/threonine-protein kinase haspin, Germ cell-specific gene 2 protein, H-haspin, Haploid germ cell-specific nuclear protein kinase, GSG2

Gene Name

HASPIN

UniProt

Q8TF76

Host

Rabbit

Reactivity

Human

Immunogen

This Haspin GSG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323~352 amino acids from the central region of human haspin.

Clonality

Polyclonal

Tissue Specificity

Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.

Applications

IHC-P, WB

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

88495 Da

Subcellular Location

Nucleus. Chromosome. Cytoplasm, cytoskeleton, spindle. Note=Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis