Anti-TRIM71 Antibody

TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71.

E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (Probable) . Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A (By similarity) . In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism (PubMed:23125361) . Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (By similarity) . Specific regulator of miRNA biogenesis. Binds to miRNA MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression (PubMed:28431233) .