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Anti-AP3D1/Ap3 Antibody

Boster Bio Anti-AP3D1/Ap3 Antibody (Catalog # A07250) . Tested in ELISA, WB applications. This antibody reacts with Human.

Product Specifications

Background

AP3D1 (also known as Delta-adaptin 3, AP-3 complex delta subunit and Delta-adaptin) is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles.

Synonyms

AP3D1, AP-3 complex subunit delta-1, Adapter-related protein complex 3 subunit delta-1, AP-3 complex subunit delta, Delta-adaptin

Gene Name

AP3D1

UniProt

O14617

Host

Goat

Reactivity

Human

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1.

Clonality

Polyclonal

Tissue Specificity

Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis.

Applications

ELISA, IP, WB

Field of Research

Adapters, Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

This affinity purified antibody is directed against human AP3D1. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and chicken based on 100% homology for the immunogen sequence. Cross-reactivity with AP3D1 protein from mouse, rat, dog and cow may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP3D1 proteins from other sources is not known.

Concentration

1.6 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

130158 MW

Gene Name Synonym

AP-3 complex subunit delta-1

Subcellular Location

Cytoplasm. Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side.

Prediction Reactivity

Chicken, Chimpanzee

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

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