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Anti-ENOB Antibody (Center)

Boster Bio Anti-ENOB Antibody (Center) (Catalog # A06845-2) . Tested in WB, IHC-P, Flow Cytometry application (s) . This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.

Synonyms

Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, MSE, Skeletal muscle enolase, ENO3

Gene Name

ENO3

UniProt

P13929

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.

Clonality

Polyclonal

Tissue Specificity

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons

Applications

WB, IHC-P, Flow Cytometry

Field of Research

Cancer, Metabolism, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Function

Glycolytic enzyme that catalyzes the conversion of 2- phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

46987 Da

Subcellular Location

Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity) .

Prediction Reactivity

Bovine, Pig

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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