Anti-Tau-tubulin kinase 2 TTBK2 Antibody

Tau tubulin kinase (TTBK2) is a serine/threonine kinase whose substrates are thought to include the tau and tubulin proteins. Mutations in the TTBK2 gene cause spinocerebellar ataxia type 11 (SCA11), a neurodegenerative disease characterized by progressive ataxia and atrophy of the cerebellum and brainstem. Recent studies have shown that increased levels of TTBK2 expression in kidney carcinoma and melanoma cell lines increases resistance to the chemotherapeutic agent Sunitinib, and that reduction of TTBK2 though the use of siRNA sensitized these cell lines to the effects of Sunitib, suggesting that TTBK2 can be used as a resistance marker as well a potential target to overcome resistance.

TTBK2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human TTBK2. The immunogen is located within the last 50 amino acids of TTBK2.

Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.

TTBK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cell projection, cilium . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytosol. Nucleus. Localizes to the transition zone in primary cilia in response to cell cycle signals that promote ciliogenesis (By similarity) . May also be present in cytosol and, at lower level in the nucleus. .