Anti-ROC2 RNF7 Antibody

ROC2 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII beta-binding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play a role in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed in heart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline induces ROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.

ROC2, GTP binding protein Roc2, Ras like protein expressed in neurons, Ras like without CAAX 2, Ras like without CAAX protein 2, RIBA, Ric (Drosophila) like, Ric like, RIN

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.

Apoptosis, Associated Proteins, Cell Biology, Epigenetics and Nuclear Signaling, Intracellular, Metabolism, Oxidative Stress, Pathways and Processes, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Redox Metabolism, Ring Finger E3 Ligase, Ubiquitin & Ubiquitin Like Modifiers, Ubiquitin E3 Enzymes

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross-reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.