Anti-Methylosome protein 50 WDR77 Antibody
Boster Bio Anti-Methylosome protein 50 WDR77 Antibody catalog # A04894. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Synonyms
Methylosome protein 50; MEP-50; Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50; WDR77; MEP50, WD45; HKMT1069, Nbla10071
Gene Name
WDR77
UniProt
Q9BQA1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human WDR77 (NP_077007.1) .
Clonality
Polyclonal
Tissue Specificity
Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
Applications
WB, ICC, IF
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452) . This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334) .
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
36724 MW
Gene Name Synonym
Methylosome protein 50
Subcellular Location
Nucleus
Protein Name
Methylosome protein 50
Contents
Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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