Anti-FAS-associated factor 1 FAF1 Antibody

Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain. Overexpression of FAF1 also decreases the basal level of NF-κB activity in transfected 293 cells, inhibits NF-κB activity induced by TNF-α , IL-1β and lipopolysaccharide, and prevents NF-κB translocation to the nucleus, suggesting that another role of FAF1 is to negatively regulate the activity of NF-κB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. Multiple differentially spliced isoforms of FAF1 are known to exist.

FAF1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAF1. The immunogen is located within the last 50 amino acids of FAF1.

Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.

Ubiquitin-binding protein (PubMed:19722279) . Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed:26842564) . Potentiates but cannot initiate FAS-induced apoptosis (By similarity) .

FAF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.