Anti-BUB3 (H274) Antibody
Boster Bio Anti-BUB3 (H274) Antibody catalog # A03118. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Synonyms
Mitotic checkpoint protein BUB3; BUB3
Gene Name
BUB3
UniProt
O43684
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 251-300 of Human BUB3.
Clonality
Polyclonal
Applications
WB, IHC, IF
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
37155 MW
Gene Name Synonym
Mitotic checkpoint protein BUB3
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore . Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. .
Protein Name
Mitotic checkpoint protein BUB3
Contents
Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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