Anti-RRM2B p53R2 Antibody

RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT) . Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.

RRM2B p53R2, RRM2B, p53R2, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein

Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.

Cancer, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Oncoproteins/Suppressors, p53 Pathway, Pathways and Processes, Tumor Suppressors

Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan; based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.