Anti-FoxO4 (phospho-S197) Antibody

Boster Bio Anti-FoxO4 (phospho-S197) Antibody catalog # A01819S197. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Forkhead box protein O4; Fork head domain transcription factor AFX1; FOXO4; AFX, AFX1, MLLT7

Gene Name

FOXO4

UniProt

P98177

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human FoxO4 around the phosphorylation site of Serine 197.

Clonality

Polyclonal

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

Applications

IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

53684 MW

Applications Notes

IHC: 1:50-1:200

Gene Name Synonym

Forkhead box protein O4

Subcellular Location

Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.