Anti-Endoribonuclease ZC3H12A ZC3H12A Antibody

ZC3H12A, also known as MCPIP, is an essential member of a family of novel CCCH-zinc finger proteins that regulate macrophage activation and may be involved in host immunity and inflammatory diseases. ZC3H12A has RNase activity that prevents some immune disorders by directly affecting the mRNA stability of interleukins such as IL-6 and IL12p40. Mice lacking the ZC3H12A gene suffered from severe anemia, and most dies within 12 weeks. Overexpression of ZC3H12A causes ER stress and induces a number of genes involved in apoptosis and autophagy, including JNK, PUMA, and beclin-1 in cardiac myoblasts, leading to cell death. ZC3H12A can also induce adipogenesis in 3T3-L1 pre-adipocytes in the absence of PPAPgamma, demonstrating the complex roles ZC3H12A plays.

ZC3H12A antibody was raised against a 17 amino acid synthetic peptide near the center of human ZC3H12A. The immunogen is located within amino acids 330 - 380 of ZC3H12A.

Expressed in heart, placenta, spleen, kidney, liver and lung (PubMed:19909337) . Expressed in leukocytes (PubMed:19909337) . Expressed in monocyte (PubMed:16574901) .

Endoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay (PubMed:19909337) . Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation- related mRNAs, such as IL6 and IL12B, during the early phase of inflammation (PubMed:26320658) . Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T- cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL) (By similarity) . Inhibits cooperatively with ZC3H12A the differentiation of helper T cells Th17 in lungs. They repress target mRNA encoding the Th17 cell-promoting factors IL6, ICOS, REL, IRF4, NFKBID and NFKBIZ. The cooperation requires RNA-binding by RC3H1 and the nuclease activity of ZC3H12A (By similarity) . Self regulates by destabilizing its own mRNA (By similarity) . Cleaves mRNA harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner (PubMed:19909337, PubMed:26320658, PubMed:26134560, PubMed:22561375) . Plays a role in the inhibition of microRNAs (miRNAs) biogenesis (PubMed:22055188) . Cleaves the terminal loop of a set of precursor miRNAs (pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs (PubMed:22055188) . Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity (PubMed:24048733) . Affects the overall ubiquitination of cellular proteins (By similarity) . Positively regulates deubiquitinase activity promoting the cleavage at 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors (TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulating macrophage-mediated inflammatory response and immune homeostasis (By similarity) . Induces also deubiquitination of the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A- targeted genes (PubMed:24048733) . Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989) . Prevents stress granule (SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock and energy deprivation (By similarity) . Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state (By similarity) . May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis (PubMed:16574901, PubMed:18364357) . Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein (APP) -dependent signaling pathway (PubMed:19185603) . Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide (LPS) by reducing I- kappa-B-kinase (IKK) -mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production (By similarity) .

ZC3H12A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.