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Anti-DNA Ligase IV LIG4 Antibody

Boster Bio Anti-DNA Ligase IV LIG4 Antibody catalog # A01685. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

DNA ligase 4;6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4

Gene Name

LIG4

UniProt

P49917

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human DNL4. AA range:591-640

Tissue Specificity

Testis, thymus, prostate and heart.

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Efficiently joins single-strand breaks in a double- stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA- dependent protein kinase complex DNA-PK to these DNA ends. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

103971 MW

Gene Name Synonym

DNA ligase 4

Subcellular Location

Nucleus.

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

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