Anti-Rad18 Antibody
Boster Bio Anti-Rad18 Antibody catalog # A01622. Tested in WB, IHC applications. This antibody reacts with Human, Rat.
Product Specifications
Synonyms
E3 ubiquitin-protein ligase RAD18;6.3.2.-; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73; RAD18; RNF73
Gene Name
RAD18
UniProt
Q9NS91
Host
Rabbit
Reactivity
Human, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD18. AA range:301-350
Applications
WB, IHC
Purification
Immunogen affinity purified
Concentration
1 mg/mL
Form
Liquid
Function
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. .
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
56223 MW
Gene Name Synonym
E3 ubiquitin-protein ligase RAD18
Subcellular Location
Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with chromatin (PubMed:25931565) . Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077) . Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607) . Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565) . .
Isotype
IgG
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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