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Anti-NOS3 (phospho-T494) Antibody

Boster Bio Anti-NOS3 (phospho-T494) Antibody catalog # A01604T494. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Synonyms

Nitric oxide synthase, endothelial;1.14.13.39; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; N

Gene Name

NOS3

UniProt

P29474

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Threonine 494.

Clonality

Polyclonal

Tissue Specificity

Platelets, placenta, liver and kidney.

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

133275 MW

Gene Name Synonym

Nitric oxide synthase, endothelial

Subcellular Location

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

Protein Name

Nitric oxide synthase, endothelial

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

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