Anti-CD33 Antibody

CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer's disease.

CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33. The immunogen is located within amino acids 180 - 230 of CD33.

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212) . Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid- bearing glycans (PubMed:7718872) . Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine- based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109) . These phosphorylations provide docking sites for the recruitment and activation of protein- tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109) . In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109) . One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323) .

CD33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.