Anti-RAD1 Antibody
Boster Bio Anti-RAD1 Antibody catalog # A01396. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Synonyms
Cell cycle checkpoint protein RAD1; hRAD1;3.1.11.2
Gene Name
RAD1
UniProt
O60671
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human RAD1.
Clonality
Polyclonal
Tissue Specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level) .
Applications
WB
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395) . The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958) . Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698) . The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698) . The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603) . Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (PubMed:9660799) .
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
31827 MW
Applications Notes
WB: 1:500-1:1000
Gene Name Synonym
Cell cycle checkpoint protein RAD1
Subcellular Location
Nucleus
Protein Name
Cell cycle checkpoint protein RAD1
Contents
Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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