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Anti-GLS Antibody

Boster Bio Anti-GLS Antibody catalog # A01272. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Glutaminase kidney isoform, mitochondrial; GLS;3.5.1.2; K-glutaminase; L-glutamine amidohydrolase; GLS; GLS1, KIAA0838

Gene Name

GLS

UniProt

O94925

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human Glutaminase (Glutaminase (GLS) ) (NP_055720.3) .

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

73461 MW

Gene Name Synonym

Glutaminase kidney isoform, mitochondrial

Subcellular Location

Isoform 1: Cytoplasm, cytosol.

Protein Name

Glutaminase kidney isoform, mitochondrial

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

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