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Anti-beta Actin ACTB Antibody

Boster Bio Anti-beta Actin ACTB Antibody (Catalog # A01263) . Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Leopard Frog.

Product Specifications

Background

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Synonyms

Beta Actin, Actin, Loading Control, beta actin, ß actin, anti-beta actin

Gene Name

ACTB

UniProt

P60709

Host

Rabbit

Reactivity

Human, Leopard frog, Mouse

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.

Clonality

Polyclonal

Applications

ELISA, IF, WB

Field of Research

Actin, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store beta-Actin Loading Control Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

41737 MW

Gene Name Synonym

Actin, cytoplasmic 1

Subcellular Location

Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Prediction Reactivity

Bovine, Goat, Guinea Pig, Hamster, Monkey, Sheep

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

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