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Anti-SPARC Antibody

Boster Bio Anti-SPARC Antibody catalog # A00862. Tested in WB, IHC-P, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine; SPARC; ON

Gene Name

SPARC

UniProt

P09486

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1) .

Clonality

Polyclonal

Applications

WB, IHC-P, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34632 MW

Gene Name Synonym

SPARC

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane

Protein Name

SPARC

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

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