Anti-Partner and localizer of BRCA2 PALB2 Antibody

The breast cancer early onset proteins BRCA1 and BRCA2 are central to the repair of DNA damage by homologous recombination and are strongly associated with inherited breast and ovarian cancer. The protein PALB2 is required for the localization of BRCA2 to sites of DNA damage and like BRCA1 and 2 is a breast cancer susceptibility gene. PALB2 is thought to function through directly binding to BRCA1, which allows the PALB2 protein to organize BRCA2 and the recombinase RAD51 at the site of DNA damage. Analysis of the BRCA1-PALB2-BRCA2-RAD51network may allow predictions to be made of the responsiveness of a particular tumor to therapeutic treatments.

PALB2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human PALB2. The immunogen is located within amino acids 940 - 990 of PALB2.

Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks (PubMed:16793542, PubMed:19423707, PubMed:19369211, PubMed:22941656, PubMed:24141787, PubMed:28319063) . Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA) (PubMed:20871615) . Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51 (PubMed:20871616) . Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination (PubMed:19369211) . Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair (PubMed:24141787) . Essential partner of BRCA2 that promotes the localization and stability of BRCA2 (PubMed:16793542) . Also enables its recombinational repair and checkpoint functions of BRCA2 (PubMed:16793542) . May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation (PubMed:16793542) . Binds DNA with high affinity for D loop, which comprises single- stranded, double-stranded and branched DNA structures (PubMed:20871616) . May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (PubMed:24485656) .

PALB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.