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Anti-GAPDH Antibody (C-term R248)

Boster Bio Anti-GAPDH Antibody (C-term R248) (Catalog # A00227-2) . Tested in WB, IF, Flow Cytometry, IHC-P application (s) . This antibody reacts with Human.

Product Specifications

Background

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.

Synonyms

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD

Gene Name

GAPDH

UniProt

P04406

Host

Rabbit

Reactivity

Human

Immunogen

This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.

Clonality

Polyclonal

Applications

WB, IF, Flow Cytometry, IHC-P

Field of Research

Cancer, Metabolism, Microbiology, Neuroscience, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Function

31705851172479431705851172479423071094230710942333215827387501

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

36053 Da

Subcellular Location

Cytoplasm, cytosol. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity) . Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261}

Prediction Reactivity

Chicken, Mouse, Pig, Rat

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Curated Selection

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