Anti-p21 (phospho-T145) CDKN1A Antibody
Boster Bio Anti-p21 (phospho-T145) CDKN1A Antibody catalog # A00145T145. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Synonyms
Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21; CDKN1A; CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Gene Name
CDKN1A
UniProt
P38936
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human p21 around the phosphorylation site of Threonine 145.
Clonality
Polyclonal
Tissue Specificity
Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
Applications
IHC
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739) .
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
18119 MW
Applications Notes
IHC: 1:50-1:200
Gene Name Synonym
Cyclin-dependent kinase inhibitor 1
Subcellular Location
Cytoplasm. Nucleus.
Protein Name
Cyclin-dependent kinase inhibitor 1
Contents
Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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