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Anti-eIF4E (phospho-S209) Antibody

Boster Bio Anti-eIF4E (phospho-S209) Antibody catalog # A00135S209. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4E; EIF4EL1, EIF4F

Gene Name

EIF4E

UniProt

P06730

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Serine 209.

Clonality

Polyclonal

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

25097 MW

Gene Name Synonym

Eukaryotic translation initiation factor 4E

Subcellular Location

Cytoplasm, P-body

Protein Name

Eukaryotic translation initiation factor 4E

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

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