Anti-Smad3 (phospho-S425) Antibody
Boster Bio Anti-Smad3 (phospho-S425) Antibody catalog # A00059S425. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Synonyms
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Gene Name
SMAD3
UniProt
P84022
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 425.
Clonality
Polyclonal
Applications
WB, IHC
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
48081 MW
Gene Name Synonym
Mothers against decapentaplegic homolog 3
Subcellular Location
Cytoplasm
Protein Name
Mothers against decapentaplegic homolog 3
Contents
Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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