Mouse TREM-1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF) ; MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40) . Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 89 102 94 97 6.05 6.2% Sample 2 219 224 232 232 226 5.53 2.4% Sample 3 892 782 872 830 844 42.21 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 89 102 94 97 6.05 6.2% Sample 1 104 89 102 94 97 6.05 6.2% Sample 2 219 224 232 232 226 5.53 2.4% Sample 2 219 224 232 232 226 5.53 2.4% Sample 3 892 782 872 830 844 42.21 5% Sample 3 892 782 872 830 844 42.21 5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4%

Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity) . .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0845-CA Rat anti-mouse TREM-1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0844-DA Biotinylated goat anti-mouse TREM-1 monoclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0845-ST Lyophilized recombinant mouse TREM-1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0845-CA Rat anti-mouse TREM-1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0844-DA Biotinylated goat anti-mouse TREM-1 monoclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0845-ST Lyophilized recombinant mouse TREM-1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0845-CA Rat anti-mouse TREM-1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0845-CA Rat anti-mouse TREM-1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0844-DA Biotinylated goat anti-mouse TREM-1 monoclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0844-DA Biotinylated goat anti-mouse TREM-1 monoclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0845-ST Lyophilized recombinant mouse TREM-1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ0845-ST Lyophilized recombinant mouse TREM-1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.062 0.109 0.182 0.292 0.611 1.107 1.755 2.234 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.062 0.109 0.182 0.292 0.611 1.107 1.755 2.234 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.062 0.109 0.182 0.292 0.611 1.107 1.755 2.234 O.D. 0.062 0.109 0.182 0.292 0.611 1.107 1.755 2.234

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.