Canine IL-17A ELISA Kit PicoKine®

Canine IL-17A ELISA Kit PicoKine® (96 Tests) . Quantitate Canine IL-17A in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO) . High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 72 95 119 97 2.09 2.2% Sample 2 500 424 389 356 417 21.45 5.1% Sample 3 1283 1546 1731 1788 1587 36.82 2.3/% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 72 95 119 97 2.09 2.2% Sample 1 102 72 95 119 97 2.09 2.2% Sample 2 500 424 389 356 417 21.45 5.1% Sample 2 500 424 389 356 417 21.45 5.1% Sample 3 1283 1546 1731 1788 1587 36.82 2.3/% Sample 3 1283 1546 1731 1788 1587 36.82 2.3/%

Synonyms

Interleukin-17A; IL-17; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; Il17a; Ctla8; Il17

Gene Name

IL17A

Gene ID

481837

UniProt

C6L8D7

Reactivity

Canine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Restricted to a subset of activated T-cells.

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 500 1283 66 483 1194 Standard deviation 4.13 15.15 59.79 4.36 25.31 47.40 CV (%) 4.1% 3.0% 4.7% 6.6% 5.2% 4.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 500 1283 66 483 1194 Standard deviation 4.13 15.15 59.79 4.36 25.31 47.40 CV (%) 4.1% 3.0% 4.7% 6.6% 5.2% 4.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 500 1283 66 483 1194 Mean (pg/ml) 102 500 1283 66 483 1194 Standard deviation 4.13 15.15 59.79 4.36 25.31 47.40 Standard deviation 4.13 15.15 59.79 4.36 25.31 47.40 CV (%) 4.1% 3.0% 4.7% 6.6% 5.2% 4.0% CV (%) 4.1% 3.0% 4.7% 6.6% 5.2% 4.0%

Sensitivity

<10 pg/mL

Function

Ligand for IL17RA (PubMed:17911633) . The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (By similarity) . Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (By similarity) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17764 MW

Specificity

Natural and recombinant canine IL-17A

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 17A

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.004 0.082 0.134 0.251 0.486 0.865 1.426 2.2 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.004 0.082 0.134 0.251 0.486 0.865 1.426 2.2 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.004 0.082 0.134 0.251 0.486 0.865 1.426 2.2 O.D. 0.004 0.082 0.134 0.251 0.486 0.865 1.426 2.2