Human Total Bcl-2 ELISA Kit PicoKine®

Bcl-2-like protein 1, also known as Bcl-X, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 121 113 78 106 2.35 2.2% Sample 2 359 449 362 386 389 23.18 6.0% Sample 3 1240 1569 1174 1581 1391 38.81 2.8/% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 121 113 78 106 2.35 2.2% Sample 1 111 121 113 78 106 2.35 2.2% Sample 2 359 449 362 386 389 23.18 6.0% Sample 2 359 449 362 386 389 23.18 6.0% Sample 3 1240 1569 1174 1581 1391 38.81 2.8/% Sample 3 1240 1569 1174 1581 1391 38.81 2.8/%

Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 359 1240 105 485 1005 Standard deviation 2.76 21.32 96.84 7.88 23.91 20.30 CV (%) 2.5% 5.9% 7.8% 7.5% 4.9% 2.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 359 1240 105 485 1005 Standard deviation 2.76 21.32 96.84 7.88 23.91 20.30 CV (%) 2.5% 5.9% 7.8% 7.5% 4.9% 2.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 359 1240 105 485 1005 Mean (pg/ml) 111 359 1240 105 485 1005 Standard deviation 2.76 21.32 96.84 7.88 23.91 20.30 Standard deviation 2.76 21.32 96.84 7.88 23.91 20.30 CV (%) 2.5% 5.9% 7.8% 7.5% 4.9% 2.0% CV (%) 2.5% 5.9% 7.8% 7.5% 4.9% 2.0%

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Isoform Bcl-X (L) : Mitochondrion inner membrane . Mitochondrion outer membrane . Mitochondrion matrix . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane ; Single-pass membrane protein ; Cytoplasmic side . After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity) . Localizes to the centrosome when phosphorylated at Ser-49. .

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.006 0.066 0.142 0.282 0.535 1.013 1.466 2.143 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.006 0.066 0.142 0.282 0.535 1.013 1.466 2.143 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.006 0.066 0.142 0.282 0.535 1.013 1.466 2.143 O.D. 0.006 0.066 0.142 0.282 0.535 1.013 1.466 2.143