Human Total DDR2 ELISA Kit PicoKine®

Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene. This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1176 1081 1035 723 1004 33.12 3.3% Sample 2 2642 3016 3175 4521 3339 223.68 6.7% Sample 3 13160 14805 18615 10787 14342 757.24 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1176 1081 1035 723 1004 33.12 3.3% Sample 1 1176 1081 1035 723 1004 33.12 3.3% Sample 2 2642 3016 3175 4521 3339 223.68 6.7% Sample 2 2642 3016 3175 4521 3339 223.68 6.7% Sample 3 13160 14805 18615 10787 14342 757.24 5.3% Sample 3 13160 14805 18615 10787 14342 757.24 5.3%

Discoidin domain-containing receptor 2; Discoidin domain receptor 2; CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD167b; DDR2; NTRKR3; TKT; TYRO10

Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level) . Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1176 2642 13160 1170 3617 10431 Standard deviation 39.16 189.43 525.08 72.42 151.55 445.40 CV (%) 3.3% 7.2% 4.0% 6.2% 4.2% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1176 2642 13160 1170 3617 10431 Standard deviation 39.16 189.43 525.08 72.42 151.55 445.40 CV (%) 3.3% 7.2% 4.0% 6.2% 4.2% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1176 2642 13160 1170 3617 10431 Mean (pg/ml) 1176 2642 13160 1170 3617 10431 Standard deviation 39.16 189.43 525.08 72.42 151.55 445.40 Standard deviation 39.16 189.43 525.08 72.42 151.55 445.40 CV (%) 3.3% 7.2% 4.0% 6.2% 4.2% 4.3% CV (%) 3.3% 7.2% 4.0% 6.2% 4.2% 4.3%

Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.052 0.124 0.193 0.307 0.528 0.91 1.427 2.02 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.052 0.124 0.193 0.307 0.528 0.91 1.427 2.02 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.052 0.124 0.193 0.307 0.528 0.91 1.427 2.02 O.D. 0.052 0.124 0.193 0.307 0.528 0.91 1.427 2.02