Monkey PD-L1 ELISA Kit PicoKine®

Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the adaptive immune system reacts to antigens that are associated with immune system activation by exogenous or endogenous danger signals.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 32 44 43 37 1.05 2.9% Sample 2 197 131 104 172 151 7.29 4.8% Sample 3 432 491 591 759 568 16.76 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 32 44 43 37 1.05 2.9% Sample 1 27 32 44 43 37 1.05 2.9% Sample 2 197 131 104 172 151 7.29 4.8% Sample 2 197 131 104 172 151 7.29 4.8% Sample 3 432 491 591 759 568 16.76 3.0% Sample 3 432 491 591 759 568 16.76 3.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 197 432 31 172 685 Standard deviation 0.87 11.09 32.27 2.24 4.76 28.56 CV (%) 3.2% 5.6% 7.5% 7.2% 2.8% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 197 432 31 172 685 Standard deviation 0.87 11.09 32.27 2.24 4.76 28.56 CV (%) 3.2% 5.6% 7.5% 7.2% 2.8% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 197 432 31 172 685 Mean (pg/ml) 27 197 432 31 172 685 Standard deviation 0.87 11.09 32.27 2.24 4.76 28.56 Standard deviation 0.87 11.09 32.27 2.24 4.76 28.56 CV (%) 3.2% 5.6% 7.5% 7.2% 2.8% 4.2% CV (%) 3.2% 5.6% 7.5% 7.2% 2.8% 4.2%

E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) . Catalyzes ufmylation of many protein, such as CD274/PD-L1, CDK5RAP3, CYB5R3, DDRGK1, EIF6, histone H4, MRE11, P4HB, PDCD1/PD-1, TRIP4, RPN1, RPS20/uS10, RPL10/uL16, RPL26/uL24, SYVN1/HRD1 and TP53/p53. As part of the UREL complex, plays a key role in ribosome recycling by catalyzing mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome. Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation: it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane. Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum. Involved in reticulophagy in response to endoplasmic reticulum stress by mediating ufmylation of proteins such as CYB5R3 and RPN1, thereby promoting lysosomal degradation of ufmylated proteins.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 25 50 100 200 400 800 1600 O.D. 0.006 0.03 0.065 0.123 0.254 0.613 1.283 1.957 Concentration (pg/ml) 0 25 50 100 200 400 800 1600 O.D. 0.006 0.03 0.065 0.123 0.254 0.613 1.283 1.957 Concentration (pg/ml) 0 25 50 100 200 400 800 1600 Concentration (pg/ml) 0 25 50 100 200 400 800 1600 O.D. 0.006 0.03 0.065 0.123 0.254 0.613 1.283 1.957 O.D. 0.006 0.03 0.065 0.123 0.254 0.613 1.283 1.957