Monkey IL-18 ELISA Kit PicoKine®

Monkey IL-18 ELISA Kit PicoKine® (96 Tests) . Quantitate Monkey IL-18 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS) . After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 8 12 10 10 0.62 6.1% Sample 2 33 26 37 38 34 1.14 3.4% Sample 3 131 111 190 128 140 7.01 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 8 12 10 10 0.62 6.1% Sample 1 11 8 12 10 10 0.62 6.1% Sample 2 33 26 37 38 34 1.14 3.4% Sample 2 33 26 37 38 34 1.14 3.4% Sample 3 131 111 190 128 140 7.01 5.0% Sample 3 131 111 190 128 140 7.01 5.0%

Synonyms

Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Il18; Igif

Gene Name

IL18

Gene ID

574151

Reactivity

Monkey

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

6.3-400 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 33 131 7 46 128 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 33 131 7 46 128 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 33 131 7 46 128 Mean (pg/ml) 11 33 131 7 46 128 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1% CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1%

Sensitivity

<1 pg/mL

Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant monkey IL-18

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-18

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-15/IL-21 family.

Protein Name

Interleukin-18

Sample Data

Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2