Monkey IL-1 beta ELISA Kit PicoKine®

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 8 11 11 10 0.57 5.5% Sample 2 46 27 44 31 37 2.74 7.4% Sample 3 115 127 180 167 147 9.06 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 8 11 11 10 0.57 5.5% Sample 1 11 8 11 11 10 0.57 5.5% Sample 2 46 27 44 31 37 2.74 7.4% Sample 2 46 27 44 31 37 2.74 7.4% Sample 3 115 127 180 167 147 9.06 6.2% Sample 3 115 127 180 167 147 9.06 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 46 115 12 43 102 Standard deviation 0.80 1.78 4.08 0.56 1.57 6.63 CV (%) 7.3% 3.9% 3.6% 4.6% 3.7% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 46 115 12 43 102 Standard deviation 0.80 1.78 4.08 0.56 1.57 6.63 CV (%) 7.3% 3.9% 3.6% 4.6% 3.7% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 46 115 12 43 102 Mean (pg/ml) 11 46 115 12 43 102 Standard deviation 0.80 1.78 4.08 0.56 1.57 6.63 Standard deviation 0.80 1.78 4.08 0.56 1.57 6.63 CV (%) 7.3% 3.9% 3.6% 4.6% 3.7% 6.5% CV (%) 7.3% 3.9% 3.6% 4.6% 3.7% 6.5%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.031 0.123 0.202 0.305 0.519 0.897 1.393 2.153 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.031 0.123 0.202 0.305 0.519 0.897 1.393 2.153 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.031 0.123 0.202 0.305 0.519 0.897 1.393 2.153 O.D. 0.031 0.123 0.202 0.305 0.519 0.897 1.393 2.153