Primate TNF-alpha ELISA Kit PicoKine®

Primate TNF-alpha ELISA Kit PicoKine® (96 Tests) . Quantitate Primate TNF-alpha in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 52 59 33 50 1.66 3.3% Sample 2 239 126 179 208 188 11.98 6.4% Sample 3 544 700 944 805 748 20.13 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 52 59 33 50 1.66 3.3% Sample 1 56 52 59 33 50 1.66 3.3% Sample 2 239 126 179 208 188 11.98 6.4% Sample 2 239 126 179 208 188 11.98 6.4% Sample 3 544 700 944 805 748 20.13 2.7% Sample 3 544 700 944 805 748 20.13 2.7%

Synonyms

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2

Gene Name

TNF

Gene ID

102139631

UniProt

P79337

Reactivity

Primate

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

Applications

ELISA

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Metabolism, Signal Transduction, TNF Superfamily, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.3-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 239 544 60 125 888 Standard deviation 4.00 11.07 32.86 3.79 7.20 39.69 CV (%) 7.2% 4.6% 6.0% 6.3% 5.8% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 239 544 60 125 888 Standard deviation 4.00 11.07 32.86 3.79 7.20 39.69 CV (%) 7.2% 4.6% 6.0% 6.3% 5.8% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 239 544 60 125 888 Mean (pg/ml) 56 239 544 60 125 888 Standard deviation 4.00 11.07 32.86 3.79 7.20 39.69 Standard deviation 4.00 11.07 32.86 3.79 7.20 39.69 CV (%) 7.2% 4.6% 6.0% 6.3% 5.8% 4.5% CV (%) 7.2% 4.6% 6.0% 6.3% 5.8% 4.5%

Sensitivity

<5 pg/mL

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25558 MW

Specificity

Natural and recombinant primate TNF-alpha

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Sequence Similarities

Belongs to the IL-15/IL-21 family.

Protein Name

Tumor necrosis factor

Sample Data

Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.044 0.089 0.151 0.253 0.451 0.772 1.28 1.9 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.044 0.089 0.151 0.253 0.451 0.772 1.28 1.9 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.044 0.089 0.151 0.253 0.451 0.772 1.28 1.9 O.D. 0.044 0.089 0.151 0.253 0.451 0.772 1.28 1.9