Feline IL-2 ELISA Kit PicoKine®

Feline IL-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Feline IL-2 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL-2, Interleukin-2, formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin-or antigen-activated T cells. In situ hybridization, the IL-2 gene is assigned to 4q26-q28. IL2 can act as a growth hormone for both B and T lymphocytes. It is useful in the study of the molecular nature of T-cell differentiation and, like interferons, augments natural killer cell activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 293 211 246 228 13.63 6.0% Sample 2 740 693 744 888 766 59.61 7.8% Sample 3 4921 4903 3989 3896 4427 302.82 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 293 211 246 228 13.63 6.0% Sample 1 162 293 211 246 228 13.63 6.0% Sample 2 740 693 744 888 766 59.61 7.8% Sample 2 740 693 744 888 766 59.61 7.8% Sample 3 4921 4903 3989 3896 4427 302.82 6.8% Sample 3 4921 4903 3989 3896 4427 302.82 6.8%

Synonyms

Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin; IL2

Gene Name

IL2

Gene ID

751114

UniProt

Q07885

Reactivity

Feline

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed in fetal brain.

Applications

ELISA

Field of Research

Adaptive Immunity, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 740 4921 276 976 3601 Standard deviation 6.17 19.61 376.95 9.72 31.04 262.15 CV (%) 3.8% 2.7% 7.7% 3.5% 3.2% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 740 4921 276 976 3601 Standard deviation 6.17 19.61 376.95 9.72 31.04 262.15 CV (%) 3.8% 2.7% 7.7% 3.5% 3.2% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 740 4921 276 976 3601 Mean (pg/ml) 162 740 4921 276 976 3601 Standard deviation 6.17 19.61 376.95 9.72 31.04 262.15 Standard deviation 6.17 19.61 376.95 9.72 31.04 262.15 CV (%) 3.8% 2.7% 7.7% 3.5% 3.2% 7.3% CV (%) 3.8% 2.7% 7.7% 3.5% 3.2% 7.3%

Sensitivity

<20 pg/mL

Function

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17654 MW

Specificity

Natural and recombinant feline IL-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-2

Subcellular Location

Secreted.

Protein Name

Interleukin-2

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.052 0.085 0.144 0.259 0.524 1.017 1.96 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.052 0.085 0.144 0.259 0.524 1.017 1.96 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.052 0.085 0.144 0.259 0.524 1.017 1.96 O.D. 0.033 0.052 0.085 0.144 0.259 0.524 1.017 1.96